A Simple Method for Elimination of False Positive Results in RT-PCR
نویسندگان
چکیده
منابع مشابه
A simple method for elimination of false positive results in RT-PCR.
Discrimination between the amplification of mRNA and contaminating genomic DNA is a common problem when performing a reverse transcriptase-polymerase chain reaction (RT-PCR). Even after treatment of the samples with DNAse, it is possible that negative controls (samples in which no reverse transcriptase was added) will give positive results. This indicates that there was amplification of DNA, wh...
متن کاملHIV false positive results and Flu vaccination
Abstract False positive results are the major problem influencing interpretation of Clinical Laboratory test. They are originated mostly in the other diseases, technical errors and the recent vaccination. The problem has been presented since 1991 is positive HIV test after influenza Vaccination (1). The False positive has been reported in Cases using Eliza, one of The most common test to screen...
متن کاملA simple RT-PCR-based strategy for screening connexin identity.
Vertebrate gap junctions are aggregates of transmembrane channels which are composed of connexin (Cx) proteins encoded by at least fourteen distinct genes in mammals. Since the same Cx type can be expressed in different tissues and more than one Cx type can be expressed by the same cell, the thorough identification of which connexin is in which cell type and how connexin expression changes afte...
متن کاملa Simple Speed Control Technique and Pulsating Torque Elimination Method in a Brushless Dc Motor
This paper presents a theoretical derivation and computer simulation of an optimal speed controller for a brushless dc motor using feedback from a linear model running in parallel with the inverter-fed model. The intent of the feedback from the linear model is to eliminate torque ripples from the inverter drive. A nonlinear model of such a motor, transformed into a linear model by a local diphe...
متن کاملFalse-positive results of plasma PCR for cytomegalovirus DNA due to delayed sample preparation.
Positive results by cytomegalovirus (CMV) PCR of plasma are considered predictive of active CMV infection in kidney allograft recipients. To assess whether contamination with leukocyte-derived CMV DNA can distort the results, aliquots of whole-blood samples from 60 CMV immunoglobulin G-positive patients with leukocyte CMV DNAemia were stored for up to 24 h at room temperature (RT) and at 4 degr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMB Reports
سال: 2002
ISSN: 1976-6696
DOI: 10.5483/bmbrep.2002.35.2.248